Lipid-mediated light activation of a mechanosensitive channel of large conductance.
نویسندگان
چکیده
This paper describes the reversible activation of a mechanosensitive channel via a light-sensitive lipid mimic. For these experiments, the mechanosensitive channel of large conductance (MscL) protein from Lactococcus lactis and Escherichia coli was reconstituted in lipid bilayers composed of 80 mol % 1,2-dioleoyl-sn-glycero-3-phosphocholine and 20 mol % di-(5-[[4-(4-butylphenyl)azo]phenoxy]pentyl)phosphate (4-Azo-5P). Light-induced isomerization of the azobenzene moiety of 4-Azo-5P from trans to cis was used to activate MscL.
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Mechanosensitive channel of large conductance.
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ورودعنوان ژورنال:
- Langmuir : the ACS journal of surfaces and colloids
دوره 20 17 شماره
صفحات -
تاریخ انتشار 2004